人肌动蛋白样蛋白6A(ACTL6A)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK5157MS

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产品名称:	Human ACTL6A(Actin Like Protein 6A) Microsample ELISA Kit
反应性:	Human
别名:	Actl6; BAF53A; Arp4; Baf53a; INO80K; BAF Complex 53 kDa Subunit; 53 kDa BRG1-associated factor A; Actin-Related Protein 4; INO80 Complex Subunit K
反应类型:	Sandwich
灵敏度:	0.056 ng/mL
标准品:	10 ng/mL
检测范围:	0.16-10 ng/mL
样本类型:	Tissue homogenates, cell lysates and other biological fluids.
反应时长:	3.5h
研究领域:	Cardiovascular biology;
Uniprot ID:	O96019
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACTL6A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACTL6A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACTL6A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACTL6A in the samples is then determined by comparing the OD of the samples to the standard curve.