小鼠髓细胞触发受体1(IREM1)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK4999MS

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产品名称:	Mouse IREM1(Immune Receptor Expressed On Myeloid Cells 1) Microsample ELISA Kit
反应性:	Mouse
别名:	CD300f; CD300LF; NKIR; IGSF13; CLM1; CD300 Antigen Like Family Member F; CMRF35-Like Molecule 1; Immunoglobulin superfamily member 13; NK inhibitory receptor
反应类型:	Sandwich
灵敏度:	0.66 ng/mL
标准品:	100 ng/mL
检测范围:	1.57-100 ng/mL
样本类型:	Tissue homogenates, cell lysates and other biological fluids.
反应时长:	3.5h
研究领域:	CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IREM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IREM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IREM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IREM1 in the samples is then determined by comparing the OD of the samples to the standard curve.