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大鼠神经介素B(NMB)酶联免疫吸附检测试剂盒
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ELK4997
规格： 价格：
48T ￥1820.00
96T ￥2600.00
96T*5 ￥11050.00
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Overview

产品名称: Rat NMB(Neuromedin B) ELISA Kit
反应性: Rat
别名: NM-B
反应类型: Competitive Inhibition
灵敏度: 43.9 pg/mL
标准品: 10000 pg/mL
检测范围: 156.25-10000 pg/mL
样本类型: Serum, plasma, other biological fluids
反应时长: 2h
研究领域: Signal transduction;Endocrinology;Neuro science;Gastroenterology;Hormone metabolism;
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NMB in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
10000.00 0.191
5000.00 0.275
2500.00 0.627
1250.00 0.998
625.00 1.189
312.50 1.439
156.25 1.724
0.00 2.236

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的NMB， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 85-97% 91%
EDTA 血浆(n=5) 78-95% 86%
Heparin 血浆(n=5) 94-105% 87%

线性

将添加有NMB的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 92-105% 82-92% 86-93% 81-94%
EDTA 血浆(n=5) 79-96% 85-99% 79-93% 89-97%
Heparin 血浆(n=5) 85-92% 85-96% 93-99% 95-102%
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