人几丁质酶1(CHIT1)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

一键复制产品信息

ELK4932MS

下载说明 ①

产品名称:	Human CHIT1(Chitinase 1) Microsample ELISA Kit
反应性:	Human
别名:	CHI3; Chitotriosidase
反应类型:	Sandwich
灵敏度:	0.11 ng/mL
标准品:	20 ng/mL
检测范围:	0.32-20 ng/mL
样本类型:	serum, plasma, tissue homogenates, cell lysates and other biological fluids
反应时长:	3.5h
研究领域:	Enzyme & Kinase;
Uniprot ID:	Q13231
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CHIT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CHIT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CHIT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CHIT1 in the samples is then determined by comparing the OD of the samples to the standard curve.