人粘膜关联上皮趋化因子(MEC)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

一键复制产品信息

ELK4889MS

下载说明 ①

产品名称:	Human MEC(Mucosae Associated Epithelia Chemokine) Microsample ELISA Kit
反应性:	Human
别名:	CCL28; SCYA28; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 28; Chemokine C-C-Motif Ligand 28; Small-inducible cytokine A28
反应类型:	Sandwich
灵敏度:	0.059 ng/mL
标准品:	10 ng/mL
检测范围:	0.16-10 ng/mL
样本类型:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
反应时长:	3.5h
研究领域:	Cytokine;Tumor immunity;Infection immunity;Dermatology;
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MEC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MEC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MEC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MEC in the samples is then determined by comparing the OD of the samples to the standard curve.