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人转移关联蛋白2(MTA2)微量上样酶联免疫吸附检测试剂盒
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ELK4886MS
规格: 价格:
48T ¥1820.00
96T ¥2600.00
96T*5 ¥11050.00

Overview

产品名称: Human MTA2(Metastasis Associated Protein 2) Microsample ELISA Kit
反应性: Human
别名: MTA1L1; PID; Metastasis-associated 1-like 1; p53 target protein in deacetylase complex; Metastasis-associated protein MTA2
反应类型: Sandwich
灵敏度: 29 pg/mL
标准品: 5000 pg/mL
检测范围: 78.13-5000 pg/mL
样本类型: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
反应时长: 3.5h
研究领域: Tumor immunity;
Uniprot ID: O94776
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MTA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MTA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MTA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MTA2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
5000.00 1.915 1.828
2500.00 1.566 1.479
1250.00 1.121 1.034
625.00 0.735 0.648
312.50 0.493 0.406
156.25 0.333 0.246
78.13 0.255 0.168
0.00 0.087 0.000

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板件的精密度。

回收率

分别往不同样本中添加已知浓度的MTA2, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 95-119% 107%
EDTA 血浆(n=5) 87-106% 97%
Heparin 血浆(n=5) 92-113% 103%

线性

将添加有MTA2的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 84-100% 84-117% 81-112% 84-94%
EDTA 血浆(n=5) 91-110% 83-95% 85-109% 91-114%
Heparin 血浆(n=5) 92-107% 82-101% 94-116% 86-116%

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