产品中心

小鼠尿皮质素1(UCN1)酶联免疫吸附检测试剂盒
一键复制产品信息
ELK4830
规格： 价格：
48T ￥1820.00
96T ￥2600.00
96T*5 ￥11050.00
下载说明 ①

Overview

产品名称: Mouse UCN1(Urocortin 1) ELISA Kit
反应性: Mouse
别名: UI; UROC; Urocortin
反应类型: Competitive Inhibition
灵敏度: 4.18 pg/mL
标准品: 1000 pg/mL
检测范围: 15.63-1000 pg/mL
样本类型: Serum, plasma and other biological fluids
反应时长: 2h
研究领域: Endocrinology;
Uniprot ID: P81615
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse UCN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse UCN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse UCN1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
1000.00 0.175
500.00 0.352
250.00 0.621
125.00 0.861
62.50 1.156
31.25 1.511
15.63 1.789
0.00 2.123

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的UCN1， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 82-95% 88%
EDTA 血浆(n=5) 85-97% 91%
Heparin 血浆(n=5) 92-105% 86%

线性

将添加有UCN1的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 92-101% 82-98% 85-96% 85-95%
EDTA 血浆(n=5) 86-95% 93-103% 87-98% 85-96%
Heparin 血浆(n=5) 85-92% 83-96% 87-98% 95-102%
关闭

在线咨询

Online consultation

  • 在线咨询
  • 技术支持

关注微信公众号

微信扫一扫立即咨询

微信电脑端咨询

微信扫一扫立即咨询

微信电脑端咨询
关闭