大鼠蛋白酶激活受体4(PAR4)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK4823MS

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产品名称:	Rat PAR4(Protease Activated Receptor 4) Microsample ELISA Kit
反应性:	Rat
别名:	F2RL3; PAR-4; Coagulation Factor II Thrombin Receptor-Like 3; Coagulation factor II receptor-like 3
反应类型:	Sandwich
灵敏度:	0.118 ng/mL
标准品:	20 ng/mL
检测范围:	0.32-20 ng/mL
样本类型:	Tissue homogenates, cell lysates and other biological fluids
反应时长:	3.5h
研究领域:	Hematology;
Uniprot ID:	Q920E0
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PAR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PAR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PAR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PAR4 in the samples is then determined by comparing the OD of the samples to the standard curve.