人紧密连接蛋白2(TJP2)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

一键复制产品信息

ELK4805MS

下载说明 ①

产品名称:	Human TJP2(Tight Junction Protein 2) Microsample ELISA Kit
反应性:	Human
别名:	X104; ZO-2; ZO2; Zona Occludens 2; Friedreich Ataxia Region Gene X104; Zona occludens protein 2; Zonula occludens protein 2
反应类型:	Sandwich
灵敏度:	0.056 ng/mL
标准品:	10 ng/mL
检测范围:	0.16-10 ng/mL
样本类型:	Tissue homogenates, cell lysates and other biological fluids
反应时长:	3.5h
研究领域:	Signal transduction;
Uniprot ID:	Q9UDY2
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TJP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TJP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TJP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TJP2 in the samples is then determined by comparing the OD of the samples to the standard curve.