人亚甲基四氢叶酸还原酶(MTHFR)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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ELK4174

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产品名称:	Human MTHFR(Methylenetetrahydrofolate Reductase) ELISA Kit
反应性:	Human
别名:	5,10-Methylenetetrahydrofolate Reductase(NADPH)
反应类型:	Sandwich
灵敏度:	22.9 pg/mL
标准品:	4000 pg/mL
检测范围:	62.5-4000 pg/mL
样本类型:	serum, plasma, tissue homogenates and other biological fluids
反应时长:	3.5h
研究领域:	Enzyme & Kinase;
Uniprot ID:	P42898
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MTHFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MTHFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MTHFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MTHFR in the samples is then determined by comparing the OD of the samples to the standard curve.