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人饥饿素(GHRL)酶联免疫吸附检测试剂盒
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ELK4080
规格： 价格：
48T ￥1820.00
96T ￥2600.00
96T*5 ￥11050.00
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Overview 文献(1)

产品名称: Human GHRL(Ghrelin) ELISA Kit
反应性: Human
别名: MTLRP; Preproghrelin; Growth Hormone Secretagogue Receptor Ligand; Appetite-regulating hormone; Growth hormone-releasing peptide; Motilin-related peptide
反应类型: Competitive Inhibition
灵敏度: 22.1 pg/mL
标准品: 5000 pg/mL
检测范围: 78.13-5000 pg/mL
样本类型: Serum, plasma and other biological fluids
反应时长: 2h
研究领域: Endocrinology;
Uniprot ID: Q9UBU6
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GHRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
5000.00 0.215
2500.00 0.310
1250.00 0.567
625.00 0.878
312.50 1.169
156.25 1.585
78.13 1.733
0.00 2.028

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的GHRL， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 83-97% 90%
EDTA 血浆(n=5) 89-103% 96%
Heparin 血浆(n=5) 86-99% 97%

线性

将添加有GHRL的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 95-103% 87-98% 85-92% 79-96%
EDTA 血浆(n=5) 86-95% 85-96% 89-100% 85-96%
Heparin 血浆(n=5) 85-92% 97-105% 92-101% 85-92%
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