人层粘连蛋白γ1(LAMC1)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK3868MS

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产品名称:	Human LAMC1(Laminin Gamma 1) Microsample ELISA Kit
反应性:	Human
别名:	LAM-C1; LAMB2; S-laminin subunit gamma; Laminin B2 chain
反应类型:	Sandwich
灵敏度:	1.39 ng/mL
标准品:	200 ng/mL
检测范围:	3.13-200 ng/mL
样本类型:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
反应时长:	3.5h
研究领域:	Metabolic pathway;Developmental science;Hepatology;Nutrition metabolism;
Uniprot ID:	P11047
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAMC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAMC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAMC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAMC1 in the samples is then determined by comparing the OD of the samples to the standard curve.