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人唾液酸结合Ig样凝集素10(SIGLEC10)微量上样酶联免疫吸附检测试剂盒
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ELK3802MS
规格: 价格:
48T ¥1820.00
96T ¥2600.00
96T*5 ¥11050.00

Overview

产品名称: Human SIGLEC10(Sialic Acid Binding Ig Like Lectin 10) Microsample ELISA Kit
反应性: Human
别名: PRO940; SLG2; Sialic Acid Binding Ig-Like Lectin 10 Ig-Like Lectin 7; Siglec-Like Gene 2
反应类型: Sandwich
灵敏度: 31 pg/mL
标准品: 5000 pg/mL
检测范围: 78.13-5000 pg/mL
样本类型: Serum, plasma, tissue homogenates and other biological fluids
反应时长: 3.5h
研究领域: Infection immunity;
Uniprot ID: Q96LC7
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SIGLEC10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIGLEC10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SIGLEC10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SIGLEC10 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
5000.00 2.198 2.115
2500.00 1.657 1.574
1250.00 1.114 1.031
625.00 0.814 0.731
312.50 0.555 0.472
156.25 0.358 0.275
78.13 0.225 0.142
0.00 0.083 0.000

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板件的精密度。

回收率

分别往不同样本中添加已知浓度的SIGLEC10, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 86-96% 91%
EDTA 血浆(n=5) 87-116% 102%
Heparin 血浆(n=5) 90-116% 103%

线性

将添加有SIGLEC10的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 84-99% 91-99% 94-103% 93-113%
EDTA 血浆(n=5) 85-119% 90-96% 88-99% 86-120%
Heparin 血浆(n=5) 91-116% 91-100% 93-98% 88-108%

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