小鼠可溶性白介素6受体(sIL-6R)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK3775MS

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产品名称:	Mouse sIL-6R(SolubleInterleukin 6 Receptor) Microsample ELISA Kit
反应性:	Mouse
别名:	IL-6R;CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80; IL6R; Interleukin 6 Receptor
反应类型:	Sandwich
灵敏度:	0.118 ng/mL
标准品:	20 ng/mL
检测范围:	0.32-20 ng/mL
样本类型:	serum, plasma, tissue homogenates and other biological fluids
反应时长:	3.5h
研究领域:	Signal transduction;CD & Adhesion molecule;Infection immunity;
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse sIL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse sIL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse sIL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse sIL-6R in the samples is then determined by comparing the OD of the samples to the standard curve.