人NADP依赖性苹果酶2(ME2)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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人NADP依赖性苹果酶2(ME2)酶联免疫吸附检测试剂盒

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ELK3646

规格：	价格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下载说明 ①

Overview

Product name:	Human ME2(Malic Enzyme 2, NADP+ Dependent, Mitochondrial) ELISA Kit
Reactivity:	Human
Alternative Names:	Malate dehydrogenase; Malic enzyme 2; Malic enzyme 2 mitochondrial; Malic enzyme 2 NAD(+) dependent mitochondrial; Malic enzyme mitochondrial; Malic enzyme NAD(+) dependent mitochondrial; MAOM_HUMAN; ME 2; ME2; mitochondrial; NAD dependent malic enzyme mitochondrial; NAD ME; NAD-dependent malic enzyme; NAD-ME; ODS1; Pyruvic malic carboxylase
Assay Type:	Sandwich
Sensitivity:	0.056 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Tissue homogenates and other biological fluids.
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;
Uniprot ID:	P23368
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ME2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ME2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ME2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ME2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	1.941	1.859
5.00	1.674	1.592
2.50	1.269	1.187
1.25	0.963	0.881
0.63	0.539	0.457
0.32	0.322	0.240
0.16	0.218	0.136
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ME2 and the recovery rates were calculated by comparing the measured value to the expected amount of ME2 in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	83-95%	89%
Heparin plasma(n=5)	80-97%	88%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ME2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.