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人金属硫蛋白1M(MT1M)酶联免疫吸附检测试剂盒
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ELK3542
规格： 价格：
48T ￥1820.00
96T ￥2600.00
96T*5 ￥11050.00
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Overview

产品名称: Human MT1M(Metallothionein 1M) ELISA Kit
反应性: Human
别名: MT1K;
反应类型: Competitive Inhibition
灵敏度: 0.96 ng/mL
标准品: 200 ng/mL
检测范围: 3.13-200 ng/mL
样本类型: Serum, plasma and other biological fluids
反应时长: 2h
研究领域: Tumor immunity;
Uniprot ID: Q8N339
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MT1M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MT1M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MT1M in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL) OD Corrected OD
200.00 0.221
100.00 0.427
50.00 0.711
25.00 0.967
12.50 1.275
6.25 1.579
3.13 1.835
0.00 2.375

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的MT1M， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 85-99% 92%
EDTA 血浆(n=5) 78-92% 85%
Heparin 血浆(n=5) 87-99% 93%

线性

将添加有MT1M的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 79-93% 89-97% 92-105% 83-92%
EDTA 血浆(n=5) 87-92% 85-94% 89-98% 85-94%
Heparin 血浆(n=5) 86-93% 92-103% 87-98% 90-101%
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