人半胱氨酸丰富血管生成诱导因子61(CYR61)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK3410MS

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产品名称:	Human CYR61(Cysteine Rich Protein, Angiogenic Inducer 61) Microsample ELISA Kit
反应性:	Human
别名:	CCN1; GIG1; IGFBP10; CCN family member 1; Insulin-like growth factor-binding protein 10
反应类型:	Sandwich
灵敏度:	24.9 pg/mL
标准品:	4000 pg/mL
检测范围:	62.5-4000 pg/mL
样本类型:	serum, plasma, tissue homogenates, cell lysates and other biological fluids
反应时长:	3.5h
研究领域:	Tumor immunity;
Uniprot ID:	O00622
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYR61. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYR61. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYR61, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYR61 in the samples is then determined by comparing the OD of the samples to the standard curve.