小鼠凝血因子Ⅻ(F12)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK3206MS

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产品名称:	Mouse F12(Coagulation Factor XII) Microsample ELISA Kit
反应性:	Mouse
别名:	FXII; HAF; Contact Factor; Hageman Factor
反应类型:	Sandwich
灵敏度:	26 pg/mL
标准品:	4000 pg/mL
检测范围:	62.5-4000 pg/mL
样本类型:	plasma
反应时长:	3.5h
研究领域:	Metabolic pathway;Hematology;
Uniprot ID:	Q80YC5
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F12 in the samples is then determined by comparing the OD of the samples to the standard curve.