人胰岛素样蛋白5(INSL5)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK3149MS

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产品名称:	Human INSL5(Insulin Like Protein 5) Microsample ELISA Kit
反应性:	Human
别名:	INSL5
反应类型:	Sandwich
灵敏度:	13.5 pg/mL
标准品:	2000 pg/mL
检测范围:	31.25-2000 pg/mL
样本类型:	serum, plasma, tissue homogenates and other biological fluids
反应时长:	3.5h
研究领域:	Endocrinology;
Uniprot ID:	Q9Y5Q6
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human INSL5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human INSL5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human INSL5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human INSL5 in the samples is then determined by comparing the OD of the samples to the standard curve.