人趋化因子C-C-基元配体14(CCL14)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK2995MS

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产品名称:	Human CCL14(Chemokine C-C-Motif Ligand 14) Microsample ELISA Kit
反应性:	Human
别名:	SCYA14; HCC-1; HCC-3; NCC-2; SCYL2; CKb1; MCIF; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 14
反应类型:	Sandwich
灵敏度:	6.3 pg/mL
标准品:	1000 pg/mL
检测范围:	15.63-1000 pg/mL
样本类型:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
反应时长:	3.5h
研究领域:	Cytokine;Tumor immunity;Infection immunity;Immune molecule;
Uniprot ID:	Q16627
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCL14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCL14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCL14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCL14 in the samples is then determined by comparing the OD of the samples to the standard curve.