人胸腺素β10(TMSb10)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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人胸腺素β10(TMSb10)酶联免疫吸附检测试剂盒

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ELK2989

规格：	价格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下载说明 ①

Overview

Product name:	Human TMSb10(Thymosin Beta 10) ELISA Kit
Reactivity:	Human
Alternative Names:	MIG12; TB10; PTMB10
Assay Type:	Competitive Inhibition
Sensitivity:	13.97 ng/mL
Standard:	2000 ng/mL
Detection Range:	31.25-2000 ng/mL
Sample Type:	Serum, plasma, other biological fluids
Assay Length:	2h
Research Area:	Endocrinology;
Uniprot ID:	P63313
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TMSb10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TMSb10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TMSb10 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
2000.00	0.215
1000.00	0.275
500.00	0.451
250.00	0.598
125.00	1.389
62.50	1.201
31.25	1.724
0.00	2.475

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TMSb10 and the recovery rates were calculated by comparing the measured value to the expected amount of TMSb10 in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	94-107%	100%
Heparin plasma(n=5)	82-95%	88%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TMSb10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.