人Ⅲ型胶原α1(COL3a1)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK2899MS

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产品名称:	Human COL3a1(Collagen Type III Alpha 1) Microsample ELISA Kit
反应性:	Human
别名:	COL3-A1; COL3A1; EDS4A; Collagen Type III Alpha 1; Ehlers-Danlos Syndrome Type IV Autosomal Dominant; Collagen Alpha-1(III)chain
反应类型:	Sandwich
灵敏度:	0.254 ng/mL
标准品:	40 ng/mL
检测范围:	0.63-40 ng/mL
样本类型:	Serum, plasma, tissue homogenates and other biological fluids
反应时长:	3.5h
研究领域:	Metabolic pathway;
Uniprot ID:	P02461
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL3a1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL3a1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL3a1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL3a1 in the samples is then determined by comparing the OD of the samples to the standard curve.