人神经纤维网蛋白1(NRP1)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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人神经纤维网蛋白1(NRP1)微量上样酶联免疫吸附检测试剂盒

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ELK2885MS

规格：	价格：
48T	￥1820.00
96T	￥2600.00
96T*5	￥11050.00

下载说明 ①

Overview

产品名称:	Human NRP1(Neuropilin 1) Microsample ELISA Kit
反应性:	Human
别名:	CD304; BDCA4; VEGF165R; Vascular endothelial cell growth factor 165 receptor
反应类型:	Sandwich
灵敏度:	27.3 pg/mL
标准品:	4000 pg/mL
检测范围:	62.5-4000 pg/mL
样本类型:	serum, plasma, tissue homogenates and other biological fluids
反应时长:	3.5h
研究领域:	Neuro science;Developmental science;
Uniprot ID:	O14786
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NRP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NRP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NRP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NRP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
4000.00	2.268	2.193
2000.00	1.663	1.588
1000.00	1.113	1.038
500.00	0.763	0.688
250.00	0.356	0.281
125.00	0.306	0.231
62.50	0.143	0.068
0.00	0.075	0.000

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板件的精密度。

回收率

Matrices listed below were spiked with certain level of recombinant NRP1 and the recovery rates were calculated by comparing the measured value to the expected amount of NRP1 in samples.

样本类型	回收率范围	平均回收率
血清(n=5)	84-114%	99%
EDTA 血浆(n=5)	83-102%	93%
Heparin 血浆(n=5)	87-97%	92%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NRP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.