大鼠间隙连接蛋白43(CX43)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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大鼠间隙连接蛋白43(CX43)微量上样酶联免疫吸附检测试剂盒

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ELK2881MS

规格：	价格：
48T	￥1820.00
96T	￥2600.00
96T*5	￥11050.00

下载说明 ①

Overview

产品名称:	Rat CX43(Connexin 43) Microsample ELISA Kit
反应性:	Rat
别名:	ODDD; GJAL; GJA1; GJ-A1; Gap junction 43 kDa heart protein; Gap Junction Protein Alpha 1; Oculodentodigital Dysplasia; Syndactyly Type III
反应类型:	Sandwich
灵敏度:	0.117 ng/mL
标准品:	20 ng/mL
检测范围:	0.32-20 ng/mL
样本类型:	tissue homogenates, cell lysates and other biological fluids
反应时长:	3.5h
研究领域:	Cardiovascular biology;Neuro science;
Uniprot ID:	P08050
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CX43. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CX43. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CX43, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CX43 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
20.00	2.102	2.008
10.00	1.542	1.448
5.00	1.129	1.035
2.50	0.882	0.788
1.25	0.488	0.394
0.63	0.398	0.304
0.31	0.142	0.048
0.00	0.094	0.000

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板件的精密度。

回收率

Matrices listed below were spiked with certain level of recombinant CX43 and the recovery rates were calculated by comparing the measured value to the expected amount of CX43 in samples.

样本类型	回收率范围	平均回收率
血清(n=5)	88-111%	100%
EDTA 血浆(n=5)	86-96%	91%
Heparin 血浆(n=5)	86-110%	98%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CX43 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.