小鼠凝血酶原片段F1+2(F1+2)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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小鼠凝血酶原片段F1+2(F1+2)酶联免疫吸附检测试剂盒

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ELK2869

规格：	价格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下载说明 ①

Overview

Product name:	Mouse F1+2(Prothrombin Fragment 1+2) ELISA Kit
Reactivity:	Mouse
Alternative Names:	F1+2
Assay Type:	Sandwich
Sensitivity:	0.111 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	Serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Hematology
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
20.00	1.990	1.922
10.00	1.542	1.474
5.00	1.043	0.975
2.50	0.780	0.712
1.25	0.509	0.441
0.63	0.305	0.237
0.32	0.223	0.155
0.00	0.068	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant F1+2 and the recovery rates were calculated by comparing the measured value to the expected amount of F1+2 in samples.

Matrix	Recovery range	Average
serum(n=5)	84-97%	90%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	77-95%	86%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F1+2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.