小鼠防御素β2(DEFb2)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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小鼠防御素β2(DEFb2)微量上样酶联免疫吸附检测试剂盒

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ELK2863MS

规格：	价格：
48T	￥1820.00
96T	￥2600.00
96T*5	￥11050.00

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Overview

产品名称:	Mouse DEFb2(Defensin Beta 2) Microsample ELISA Kit
反应性:	Mouse
别名:	B-DF2; DEFB2; BD2; DEF-B2; HBD2; DEFB102; HBD-2; SAP1; DEFB4A; DEFB4; Skin-Antimicrobial Peptide 1
反应类型:	Sandwich
灵敏度:	5.6 pg/mL
标准品:	1000 pg/mL
检测范围:	15.63-1000 pg/mL
样本类型:	serum, plasma, tissue homogenates, cell lysates and other biological fluids
反应时长:	3.5h
研究领域:	Infection immunity;
Uniprot ID:	P82020
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DEFb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DEFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DEFb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DEFb2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	1.943	1.847
500.00	1.700	1.604
250.00	1.149	1.053
125.00	0.742	0.646
62.50	0.553	0.457
31.25	0.302	0.206
15.63	0.234	0.138
0.00	0.096	0.000

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板件的精密度。

回收率

Matrices listed below were spiked with certain level of recombinant DEFb2 and the recovery rates were calculated by comparing the measured value to the expected amount of DEFb2 in samples.

样本类型	回收率范围	平均回收率
血清(n=5)	87-101%	94%
EDTA 血浆(n=5)	92-115%	104%
Heparin 血浆(n=5)	86-111%	99%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DEFb2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.