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人摄食抑制因子1(NES1)酶联免疫吸附检测试剂盒
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ELK2828
规格： 价格：
48T ￥1820.00
96T ￥2600.00
96T*5 ￥11050.00
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Overview

产品名称: Human NES1(Nesfatin 1) ELISA Kit
反应性: Human
别名: Nesfatin-1
反应类型: Competitive Inhibition
灵敏度: 225.6 pg/mL
标准品: 50000 pg/mL
检测范围: 781.25-50000 pg/mL
样本类型: serum, plasma and other biological fluids
反应时长: 2h
研究领域: Glycolipid metabolism
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NES1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NES1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NES1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
50000.00 0.175
25000.00 0.367
12500.00 0.577
6250.00 0.833
3125.00 1.197
1562.50 1.578
781.25 1.755
0.00 2.101

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的NES1， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 92-106% 99%
EDTA 血浆(n=5) 88-103% 95%
Heparin 血浆(n=5) 80-97% 88%

线性

将添加有NES1的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 95-103% 79-97% 96-105% 95-103%
EDTA 血浆(n=5) 85-92% 79-96% 95-103% 87-98%
Heparin 血浆(n=5) 85-92% 89-101% 87-98% 85-92%
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