小鼠促甲状腺素释放激素(TRH)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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小鼠促甲状腺素释放激素(TRH)酶联免疫吸附检测试剂盒

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ELK2642

规格：	价格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下载说明 ①

Overview

Product name:	Mouse TRH(Thyrotropin Releasing Hormone) ELISA Kit
Reactivity:	Mouse
Alternative Names:	TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor
Assay Type:	Competitive Inhibition
Sensitivity:	2.69 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Endocrinology;Neuro science;Hepatology;Hormone metabolism;
Uniprot ID:	Q62361
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TRH in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
500.00	0.191
250.00	0.336
125.00	0.527
62.50	0.899
31.25	1.157
15.63	1.537
7.82	1.624
0.00	2.028

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TRH and the recovery rates were calculated by comparing the measured value to the expected amount of TRH in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	80-97%	88%
Heparin plasma(n=5)	78-92%	85%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.