大鼠碱性鞘磷脂磷酸二酯酶(Alk-Smase)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

产品中心

大鼠碱性鞘磷脂磷酸二酯酶(Alk-Smase)酶联免疫吸附检测试剂盒

一键复制产品信息

ELK2629

规格：	价格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下载说明 ①

Overview

Product name:	Rat Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit
Reactivity:	Rat
Alternative Names:	ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase
Assay Type:	Sandwich
Sensitivity:	0.046 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Tissue homogenates and other biological fluids.
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Neuro science;
Uniprot ID:	Q5EZ72
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	2.035	1.946
5.00	1.533	1.444
2.50	1.096	1.007
1.25	0.807	0.718
0.63	0.538	0.449
0.32	0.329	0.240
0.16	0.206	0.117
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Alk-Smase and the recovery rates were calculated by comparing the measured value to the expected amount of Alk-Smase in samples.

Matrix	Recovery range	Average
serum(n=5)	78-95%	86%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	78-93%	85%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alk-Smase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.