小鼠免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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小鼠免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)酶联免疫吸附检测试剂盒

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ELK2523

规格：	价格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下载说明 ①

Overview

Product name:	Mouse Tie1(Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1) ELISA Kit
Reactivity:	Mouse
Alternative Names:	JTK14; Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domains 1; Tyrosine-protein kinase receptor Tie-1
Assay Type:	Sandwich
Sensitivity:	0.266 ng/mL
Standard:	40 ng/mL
Detection Range:	0.63-40 ng/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Tumor immunity;Infection immunity;
Uniprot ID:	Q06806
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Tie1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Tie1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Tie1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Tie1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
40.00	2.119	2.033
20.00	1.695	1.609
10.00	1.215	1.129
5.00	0.661	0.575
2.50	0.457	0.371
1.25	0.335	0.249
0.63	0.187	0.101
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Tie1 and the recovery rates were calculated by comparing the measured value to the expected amount of Tie1 in samples.

Matrix	Recovery range	Average
serum(n=5)	92-105%	98%
EDTA plasma(n=5)	84-96%	90%
Heparin plasma(n=5)	80-95%	87%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tie1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.