人神经调节素1(NRG1)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

产品中心

人神经调节素1(NRG1)微量上样酶联免疫吸附检测试剂盒

一键复制产品信息

ELK2232MS

规格：	价格：
48T	￥1820.00
96T	￥2600.00
96T*5	￥11050.00

下载说明 ①

Overview

产品名称:	Human NRG1(Neuregulin 1) Microsample ELISA Kit
反应性:	Human
别名:	ARIA; GGF; HGL; HRG; HRGA; NDF; SMDF; Acetylcholine receptor-inducing activity; Glial growth factor; Heregulin; Neu differentiation factor; Sensory and motor neuron-derived factor
实验方法:	Sandwich
灵敏度:	0.051 ng/mL
标准品:	10 ng/mL
检测范围:	0.16-10 ng/mL
样本类型:	Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
反应时长:	3.5h
研究领域:	Signal transduction;Neuro science;
Uniprot ID:	Q02297
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NRG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NRG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NRG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NRG1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	2.126	2.038
5.00	1.528	1.440
2.50	1.132	1.044
1.25	0.965	0.877
0.63	0.528	0.440
0.31	0.373	0.285
0.16	0.194	0.106
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NRG1 and the recovery rates were calculated by comparing the measured value to the expected amount of NRG1 in samples.

Matrix	Recovery range	Average
serum(n=5)	88-113%	101%
EDTA plasma(n=5)	81-119%	100%
Heparin plasma(n=5)	93-107%	100%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NRG1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.