小鼠核因子κB2(NFkB2)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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小鼠核因子κB2(NFkB2)微量上样酶联免疫吸附检测试剂盒

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ELK2209MS

规格：	价格：
48T	￥1820.00
96T	￥2600.00
96T*5	￥11050.00

下载说明 ①

Overview

产品名称:	Mouse NFkB2(Nuclear Factor Kappa B2) Microsample ELISA Kit
反应性:	Mouse
别名:	LYT-10; H2TF1; Lymphocyte translocation chromosome 10; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2(p49/P100); Nuclear factor NF-kappa-B p52
实验方法:	Sandwich
灵敏度:	0.057 ng/mL
标准品:	10 ng/mL
检测范围:	0.16-10 ng/mL
样本类型:	Tissue homogenates, cell lysates and other biological fluids.
反应时长:	3.5h
研究领域:	Signal transduction;
Uniprot ID:	Q9WTK5
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFkB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFkB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFkB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFkB2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	2.049	1.946
5.00	1.735	1.632
2.50	1.286	1.183
1.25	0.832	0.729
0.63	0.572	0.469
0.31	0.341	0.238
0.16	0.246	0.143
0.00	0.103	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NFkB2 and the recovery rates were calculated by comparing the measured value to the expected amount of NFkB2 in samples.

Matrix	Recovery range	Average
serum(n=5)	81-109%	95%
EDTA plasma(n=5)	85-105%	95%
Heparin plasma(n=5)	80-103%	92%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NFkB2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.