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人神经降压素(NT)酶联免疫吸附检测试剂盒
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ELK1802
规格: 价格:
48T ¥1820.00
96T ¥2600.00
96T*5 ¥11050.00

Overview

产品名称: Human NT(Neurotensin) ELISA Kit
反应性: Human
别名: NTS; NTS1; NmN-125; NN; Tail peptide Neuromedin N
反应类型: Competitive Inhibition
灵敏度: 14.2 pg/mL
标准品: 3000 pg/mL
检测范围: 46.88-3000 pg/mL
样本类型: serum, plasma and other biological fluids
反应时长: 2h
研究领域: Reproductive science;Neuro science;Hormone metabolism;
Uniprot ID: P30990
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
3000.00 0.235
1500.00 0.389
750.00 0.655
375.00 0.856
187.50 1.225
93.75 1.596
46.88 1.761
0.00 2.325

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的NT, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 95-108% 101%
EDTA 血浆(n=5) 83-97% 90%
Heparin 血浆(n=5) 80-97% 88%

线性

将添加有NT的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 90-99% 87-103% 78-91% 83-97%
EDTA 血浆(n=5) 85-102% 83-96% 95-102% 87-98%
Heparin 血浆(n=5) 89-97% 86-102% 94-106% 85-97%
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