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人甘丙肽(GAL)酶联免疫吸附检测试剂盒
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ELK1756
规格: 价格:
48T ¥1820.00
96T ¥2600.00
96T*5 ¥11050.00

Overview

产品名称: Human GAL(Galanin) ELISA Kit
反应性: Human
别名: GALN; GLNN; GMAP; Galanin Prepropeptide; Galanin-Message-Associated Peptide
反应类型: Competitive Inhibition
灵敏度: 4.83 pg/mL
标准品: 1000 pg/mL
检测范围: 15.63-1000 pg/mL
样本类型: Serum, plasma, cell lysates, cell culture supernates and other biological fluids
反应时长: 2h
研究领域: Metabolic pathway;Endocrinology;Reproductive science;Neuro science;Hepatology;Gastroenterology;Hormone metabolism;
Uniprot ID: P22466
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GAL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GAL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GAL in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
1000.00 0.189
500.00 0.397
250.00 0.583
125.00 0.857
62.50 1.152
31.25 1.511
15.63 1.762
0.00 2.123

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的GAL, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 82-98% 90%
EDTA 血浆(n=5) 84-98% 91%
Heparin 血浆(n=5) 80-96% 88%

线性

将添加有GAL的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 85-94% 87-105% 92-101% 79-90%
EDTA 血浆(n=5) 85-102% 82-96% 87-98% 78-92%
Heparin 血浆(n=5) 88-103% 86-97% 87-102% 89-101%
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