人爱帕琳肽12(AP12)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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ELK1635

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产品名称:	Human AP12(Apelin 12) ELISA Kit
反应性:	Human
别名:	APLN12
反应类型:	Competitive Inhibition
灵敏度:	25.28 pg/mL
标准品:	6000 pg/mL
检测范围:	93.75-6000 pg/mL
样本类型:	Serum, plasma, cell lysates, cell culture supernates and other biological fluids
反应时长:	2h
研究领域:	Metabolic pathway;Endocrinology;Cardiovascular biology;Gastroenterology;Hormone metabolism;
检测原理:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP12 in the samples is then determined by comparing the OD of the samples to the standard curve.