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大鼠胰淀素(IAPP)酶联免疫吸附检测试剂盒
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ELK1602
规格： 价格：
48T ￥1820.00
96T ￥2600.00
96T*5 ￥11050.00
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产品名称: Rat IAPP(Islet Amyloid Polypeptide) ELISA Kit
反应性: Rat
别名: IAP; DAP; Amylin; Insulinoma Amyloid Polypeptide; Diabetes-associated peptide
反应类型: Competitive Inhibition
灵敏度: 2.73 pg/mL
标准品: 500 pg/mL
检测范围: 7.82-500 pg/mL
样本类型: serum, plasma, cell lysates, cell culture supernates and other biological fluids
反应时长: 2h
研究领域: Metabolic pathway;Endocrinology;Hepatology;
Uniprot ID: P12969
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat IAPP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IAPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IAPP in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
500.00 0.221
250.00 0.387
125.00 0.689
62.50 0.913
31.25 1.225
15.63 1.596
7.82 1.871
0.00 2.316

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的IAPP， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 85-97% 91%
EDTA 血浆(n=5) 82-94% 88%
Heparin 血浆(n=5) 89-107% 98%

线性

将添加有IAPP的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 95-104% 97-105% 82-95% 89-97%
EDTA 血浆(n=5) 86-95% 82-93% 97-106% 89-102%
Heparin 血浆(n=5) 95-104% 82-96% 92-103% 90-99%
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