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人胃泌素抑制肽(GIP)酶联免疫吸附检测试剂盒
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ELK1580
规格: 价格:
48T ¥1820.00
96T ¥2600.00
96T*5 ¥11050.00

Overview

产品名称: Human GIP(Gastric Inhibitory Polypeptide) ELISA Kit
反应性: Human
别名: Incretin hormone;  Glucose Dependent Insulinotropic Peptide
反应类型: Competitive Inhibition
灵敏度: 23.5 pg/mL
标准品: 5000 pg/mL
检测范围: 78.13-5000 pg/mL
样本类型: serum, plasma, cell lysates, cell culture supernates and other biological fluids
反应时长: 2h
研究领域: Endocrinology;Gastroenterology;Hormone metabolism;
Uniprot ID: P09681
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GIP in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
5000.00 0.211
2500.00 0.352
1250.00 0.557
625.00 0.861
312.50 1.152
156.25 1.511
78.13 1.755
0.00 2.132

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的GIP, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 90-105% 97%
EDTA 血浆(n=5) 85-97% 91%
Heparin 血浆(n=5) 96-107% 101%

线性

将添加有GIP的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 79-85% 87-96% 82-95% 89-103%
EDTA 血浆(n=5) 95-102% 96-104% 87-98% 95-107%
Heparin 血浆(n=5) 82-94% 85-96% 78-90% 91-99%
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