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大鼠β-骨胶原交联(bCTx)酶联免疫吸附检测试剂盒
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ELK1546
规格: 价格:
48T ¥1820.00
96T ¥2600.00
96T*5 ¥11050.00

Overview

产品名称: Rat bCTx(Beta-Crosslaps) ELISA Kit
反应性: Rat
别名: Beta CTx; b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction
反应类型: Competitive Inhibition
灵敏度: 39.1 pg/mL
标准品: 8000 pg/mL
检测范围: 125-8000 pg/mL
样本类型: serum, plasma, cell lysates, cell culture supernates and other biological fluids
反应时长: 2h
研究领域: Bone metabolism;
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat bCTx. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat bCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat bCTx in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
8000.00 0.175
4000.00 0.367
2000.00 0.583
1000.00 0.857
500.00 1.156
250.00 1.511
125.00 1.799
0.00 2.115

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的bCTx, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 82-96% 89%
EDTA 血浆(n=5) 78-90% 84%
Heparin 血浆(n=5) 85-97% 91%

线性

将添加有bCTx的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 85-99% 82-96% 92-101% 82-90%
EDTA 血浆(n=5) 79-92% 80-93% 81-98% 89-102%
Heparin 血浆(n=5) 87-98% 92-104% 82-96% 95-102%
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