小鼠肌酸激酶MB同工酶(CKMB)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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小鼠肌酸激酶MB同工酶(CKMB)酶联免疫吸附检测试剂盒

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ELK1286

规格：	价格：
48T	￥1680.00
96T	￥2400.00
96T*5	￥10200.00

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Overview 文献(13)

Product name:	Mouse CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit
Reactivity:	Mouse
Alternative Names:	CK2; CK-MB; Creatine Kinase,Muscle/Brain
Assay Type:	Sandwich
Sensitivity:	0.28 ng/mL
Standard:	50 ng/mL
Detection Range:	0.79-50 ng/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Cardiovascular biology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CKMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CKMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CKMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CKMB in the samples is then determined by comparing the OD of the samples to the standard curve.

Cardiovascular Diabetology

Melatonin attenuates diabetic cardiomyopathy by increasing autophagy of cardiomyocytes via regulation of VEGF-B/GRP78/PERK signaling pathway

日期：2024-01-09 IF：9.3
Frontiers in Cellular and Infection Microbiology

New insights of necroptosis and immune infiltration in sepsis-induced myocardial dysfunction from bioinformatics analysis through RNA-seq in mice

日期：2022-12-15 IF：6.073
Chemico-Biological Interactions

Zn2+ regulates mitochondrial DNA efflux to inhibit AIM2-mediated ZBP1-PANoptosome pathway and alleviate septic myocardial injury

日期：2025-06-16 IF：4.7
Cellular Signalling

Methylophiopogonanone A alleviates diabetic cardiomyopathy via inhibiting JNK1 signaling

日期：2025-04-17 IF：4.4
Toxicology and Applied Pharmacology

A novel histone deacetylase inhibitor Se-SAHA attenuates isoproterenol-induced heart failure via antioxidative stress and autophagy inhibition

日期：2024-05-23 IF：3.8
BMC Infectious Diseases

Integrated bioinformatics and experiment validation reveal cuproptosis-related biomarkers and therapeutic targets in sepsis-induced myocardial dysfunction

日期：2025-04-17 IF：3.4
Saudi Pharmaceutical Journal

Ceftriaxone and MC-100093 mitigate fentanyl-induced cardiac injury in mice: Preclinical investigation of its underlying molecular mechanisms

日期：2024-07-27 IF：3
Infection and Drug Resistance

Validation of Senescence of the Role of ATM/P53 Pathway in Myocardial Senescence in Mice with Sepsis

日期：2025-05-13 IF：2.9
Fundamental & Clinical Pharmacology

Exogenous fetuin-A protects against sepsis-induced myocardial injury by inhibiting oxidative stress and inflammation in mice

日期：2023-01-16 IF：2.747
BMC Cardiovascular Disorders

Polyethylene glycol loxenatide protects diabetic kidneys by inhibiting GRP78/PERK/ eIF2α pathway, and improves cardiac injury by suppressing TLR4/NF-κB infammatory pathway

日期：2025-01-17 IF：2
BMC Cardiovascular Disorders

Polyethylene glycol loxenatide protects diabetic kidneys by inhibiting GRP78/PERK/ eIF2α pathway, and improves cardiac injury by suppressing TLR4/NF-κB infammatory pathway

日期：2025-01-17 IF：2
BMC Cardiovascular Disorders

Polyethylene glycol loxenatide protects diabetic kidneys by inhibiting GRP78/PERK/ eIF2α pathway, and improves cardiac injury by suppressing TLR4/NF-κB infammatory pathway

日期：2025-01-17 IF：2
BMC Cardiovascular Disorders

Polyethylene glycol loxenatide protects diabetic kidneys by inhibiting GRP78/PERK/ eIF2α pathway, and improves cardiac injury by suppressing TLR4/NF-κB infammatory pathway

日期：2025-01-17 IF：2

标准曲线

Concentration (ng/mL)	OD	Corrected OD
50.00	1.916	1.829
25.00	1.394	1.307
12.50	0.993	0.906
6.25	0.796	0.709
3.13	0.473	0.386
1.57	0.327	0.240
0.79	0.214	0.127
0.00	0.087	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CKMB and the recovery rates were calculated by comparing the measured value to the expected amount of CKMB in samples.

Matrix	Recovery range	Average
serum(n=5)	84-97%	90%
EDTA plasma(n=5)	82-94%	88%
Heparin plasma(n=5)	81-97%	89%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CKMB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.