小鼠2,3-二磷酸甘油酸(2,3-DPG)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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ELK11892

产品名称:	Mouse 2,3-DPG(2,3-Diphosphoglycerate) ELISA Kit
反应性:	Mouse
别名:	2,3DPG
反应类型:	Sandwich
灵敏度:	0.13 μmol/mL
标准品:	20 μmol/mL
检测范围:	0.32-20 μmol/mL
样本类型:	Serum, plasma, tissue homogenates, cell lysates and other biological fluids
反应时长:	3.5h
研究领域:	Metabolic pathway
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse 2,3-DPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 2,3-DPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse 2,3-DPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 2,3-DPG in the samples is then determined by comparing the OD of the samples to the standard curve.