人甲型流感病毒H3N2(H3N2)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK11850

产品名称:	Human H3N2(Influenza A H3N2) ELISA Kit
反应性:	Human
别名:	Influenza A H3N2 (A/Hong Kong/1/1968) ;Nucleoprotein ;NP
反应类型:	Sandwich
灵敏度:	3.7 pg/mL
标准品:	5000 pg/mL
检测范围:	78.13-5000 pg/mL
样本类型:	Serum, plasma
反应时长:	3.5h
研究领域:	Microbiology;
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human H3N2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human H3N2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human H3N2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human H3N2 in the samples is then determined by comparing the OD of the samples to the standard curve.