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小鼠醛固酮(ALD)酶联免疫吸附检测试剂盒
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ELK11272
规格： 价格：
48T ￥1960.00
96T ￥2800.00
96T*5 ￥11900.00
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Overview

产品名称: Mouse ALD(Aldosterone) ELISA Kit
反应性: Mouse
别名: ALD
反应类型: Competitive Inhibition
灵敏度: 9.41 pg/mL
标准品: 2000 pg/mL
检测范围: 31.25-2000 pg/mL
样本类型: serum, plasma and other biological fluids
反应时长: 2h
研究领域: Metabolic pathway;Endocrinology;Hormone metabolism;
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse ALD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ALD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ALD in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
2000.00 0.189
1000.00 0.427
500.00 0.596
250.00 0.933
125.00 1.169
62.50 1.583
31.25 1.799
0.00 2.213

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的ALD， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 83-97% 90%
EDTA 血浆(n=5) 80-92% 86%
Heparin 血浆(n=5) 85-99% 92%

线性

将添加有ALD的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 79-95% 96-105% 85-96% 90-99%
EDTA 血浆(n=5) 82-96% 95-102% 87-98% 81-97%
Heparin 血浆(n=5) 86-93% 93-101% 85-98% 90-101%
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