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小鼠6酮前列腺素F1α(6ketoPGF1α)酶联免疫吸附检测试剂盒
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ELK11243
规格： 价格：
48T ￥1820.00
96T ￥2600.00
96T*5 ￥11050.00
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Overview

产品名称: Mouse 6ketoPGF1α(6-keto-PGF1 alpha) ELISA Kit
反应性: Mouse
别名: 6-keto-PGF1 alpha; 6ketoPGF1α
反应类型: Competitive Inhibition
灵敏度: 2.4 pg/mL
标准品: 500 pg/mL
检测范围: 7.82-500 pg/mL
样本类型: Serum, plasma and other biological fluids.
反应时长: 2h
研究领域: Neuro science;
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 6ketoPGF1α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 6ketoPGF1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 6ketoPGF1α in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
500.00 0.185
250.00 0.336
125.00 0.557
62.50 0.899
31.25 1.236
15.63 1.537
7.82 1.733
0.00 2.213

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的6ketoPGF1α， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 90-105% 97%
EDTA 血浆(n=5) 88-102% 95%
Heparin 血浆(n=5) 94-107% 100%

线性

将添加有6ketoPGF1α的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 82-90% 92-101% 85-92% 87-103%
EDTA 血浆(n=5) 95-103% 97-105% 88-97% 97-105%
Heparin 血浆(n=5) 87-94% 93-101% 88-95% 96-101%
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