大鼠可溶性末端补体复合物sC5b-9(sC5b-9)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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ELK11154

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产品名称:	Rat sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
反应性:	Rat
别名:	MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9
反应类型:	Sandwich
灵敏度:	13.32 ng/mL
标准品:	2000 ng/mL
检测范围:	31.25-2000 ng/mL
样本类型:	serum, plasma and other biological fluids
反应时长:	3.5h
研究领域:	Signal transduction;Metabolic pathway;Apoptosis;Infection immunity;
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.