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大鼠去甲肾上腺素(NE)酶联免疫吸附检测试剂盒
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ELK10884
规格： 价格：
48T ￥1820.00
96T ￥2600.00
96T*5 ￥11050.00
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产品名称: Rat NE(Noradrenaline/Norepinephrine) ELISA Kit
反应性: Rat
别名: NA; NAd; Norepinephrine; Norepi
反应类型: Competitive Inhibition
灵敏度: 26.2 pg/mL
标准品: 5000 pg/mL
检测范围: 78.13-5000 pg/mL
样本类型: serum, plasma, cell lysates, cell culture supernates and other biological fluids
反应时长: 2h
研究领域: Signal transduction;Infection immunity;Neuro science;Hormone metabolism;
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NE in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
5000.00 0.175
2500.00 0.352
1250.00 0.655
625.00 0.856
312.50 1.225
156.25 1.596
78.13 1.813
0.00 2.211

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的NE， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 87-95% 91%
EDTA 血浆(n=5) 93-107% 100%
Heparin 血浆(n=5) 87-99% 93%

线性

将添加有NE的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 85-101% 87-103% 84-92% 78-90%
EDTA 血浆(n=5) 91-98% 83-101% 87-98% 95-102%
Heparin 血浆(n=5) 85-96% 95-104% 93-102% 79-94%
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