牛胰岛素样生长因子结合蛋白4(IGFBP4)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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ELK10693

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产品名称:	Cattle IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit
反应性:	Cattle
别名:	BP4; HT29-IGFBP; IBP4
反应类型:	Competitive Inhibition
灵敏度:	0.56 ng/mL
标准品:	100 ng/mL
检测范围:	1.57-100 ng/mL
样本类型:	Serum, plasma and other biological fluids
反应时长:	2h
研究领域:	Apoptosis;Tumor immunity;
Uniprot ID:	Q05716
检测原理:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle IGFBP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IGFBP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IGFBP4 in the samples is then determined by comparing the OD of the samples to the standard curve.