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小鼠Salusin肽α(SALa)酶联免疫吸附检测试剂盒
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ELK10651
规格: 价格:
48T ¥1820.00
96T ¥2600.00
96T*5 ¥11050.00

Overview

产品名称: Mouse SALa(Salusin Alpha) ELISA Kit
反应性: Mouse
别名: SALa
反应类型: Competitive Inhibition
灵敏度: 0.051 ng/mL
标准品: 10 ng/mL
检测范围: 0.16-10 ng/mL
样本类型: serum, plasma and other biological fluids
反应时长: 2h
研究领域: Infection immunity;Endocrinology;Cardiovascular biology;Neuro science;
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse SALa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SALa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SALa in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL) OD Corrected OD
10.00 0.227
5.00 0.357
2.50 0.596
1.25 0.895
0.63 1.169
0.32 1.585
0.16 1.715
0.00 2.112

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的SALa, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 88-99% 93%
EDTA 血浆(n=5) 82-95% 88%
Heparin 血浆(n=5) 80-97% 101%

线性

将添加有SALa的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 85-101% 78-96% 77-91% 85-93%
EDTA 血浆(n=5) 87-102% 96-105% 85-93% 89-97%
Heparin 血浆(n=5) 82-96% 85-101% 87-102% 89-95%
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