小鼠β淀粉样蛋白1-42(Ab1-42)微量上样酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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小鼠β淀粉样蛋白1-42(Ab1-42)微量上样酶联免疫吸附检测试剂盒

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ELK10615MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

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Overview

Product name:	Mouse Ab1-42(Amyloid Beta Peptide 1-42) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	Amyloid beta 42(ABeta 42); amyloid beta peptide 1-42; Aβ1-42,amyloid βpeptide 1-42
Assay Type:	Sandwich
Sensitivity:	4.34 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Neuro science;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.101	2.011
500.00	1.676	1.586
250.00	1.261	1.171
125.00	0.843	0.753
62.50	0.445	0.355
31.25	0.361	0.271
15.63	0.222	0.132
0.00	0.090	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Ab1-42 and the recovery rates were calculated by comparing the measured value to the expected amount of Ab1-42 in samples.

Matrix	Recovery range	Average
serum(n=5)	85-113%	99%
EDTA plasma(n=5)	92-103%	98%
Heparin plasma(n=5)	92-112%	102%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ab1-42 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.