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小鼠纤维蛋白肽A(FPA)酶联免疫吸附检测试剂盒
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ELK10608
规格: 价格:
48T ¥1820.00
96T ¥2600.00
96T*5 ¥11050.00

Overview

产品名称: Mouse FPA(Fibrinopeptide A) ELISA Kit
反应性: Mouse
别名: FP-A
反应类型: Competitive Inhibition
灵敏度: 0.45 ng/mL
标准品: 100 ng/mL
检测范围: 1.57-100 ng/mL
样本类型: Serum, plasma, cell lysates, cell culture supernates and other biological fluids
反应时长: 2h
研究领域: Signal transduction;Hematology;
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse FPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FPA in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL) OD Corrected OD
100.00 0.182
50.00 0.387
25.00 0.692
12.50 0.913
6.25 1.139
3.13 1.596
1.57 1.813
0.00 2.325

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的FPA, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 90-105% 97%
EDTA 血浆(n=5) 82-95% 88%
Heparin 血浆(n=5) 95-107% 96%

线性

将添加有FPA的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 85-102% 84-99% 89-97% 85-93%
EDTA 血浆(n=5) 86-96% 93-104% 89-97% 85-94%
Heparin 血浆(n=5) 93-105% 89-96% 79-98% 81-92%
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