产品中心

人爱帕琳肽36(AP36)酶联免疫吸附检测试剂盒
一键复制产品信息
ELK10383
规格： 价格：
48T ￥1820.00
96T ￥2600.00
96T*5 ￥11050.00
下载说明 ①

Overview

产品名称: Human AP36(Apelin 36) ELISA Kit
反应性: Human
别名: APLN36
反应类型: Competitive Inhibition
灵敏度: 0.071 ng/mL
标准品: 10 ng/mL
检测范围: 0.16-10 ng/mL
样本类型: Serum, plasma and other biological fluids
反应时长: 2h
研究领域: Metabolic pathway;Endocrinology;Gastroenterology;
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP36. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP36. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP36 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL) OD Corrected OD
10.00 0.212
5.00 0.357
2.50 0.611
1.25 0.833
0.63 1.156
0.32 1.511
0.16 1.775
0.00 2.123

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的AP36， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 85-97% 91%
EDTA 血浆(n=5) 83-99% 95%
Heparin 血浆(n=5) 85-97% 91%

线性

将添加有AP36的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 79-91% 87-96% 92-101% 82-95%
EDTA 血浆(n=5) 95-102% 83-96% 97-105% 85-97%
Heparin 血浆(n=5) 93-105% 85-97% 90-99% 82-101%
关闭

在线咨询

Online consultation

  • 在线咨询
  • 技术支持

关注微信公众号

微信扫一扫立即咨询

微信电脑端咨询

微信扫一扫立即咨询

微信电脑端咨询
关闭